Publications
Publications
(★=selectred publication)
39. Date Y, Sasazawa Y, Kitagawa M, Gejima K, Suzuki A, Saya H, Kida Y, Imoto M, Itakura E, Hattori N, Saiki S.
Novel autophagy inducers by accelerating lysosomal clustering against Parkinson's disease
Elife. 2024 Jul 3:13:e98649. doi: 10.7554/eLife.98649.
38. Zhang H, Ishii K, Shibata T, Ishii S, Hirao M, Lu Z, Takamura R, Kitano S, Miyachi H, Kageyama R, Itakura E, Kobayashi T.
Fluctuation of lysosomal protein degradation in neural stem cells of the postnatal mouse brain
Development. 2024 Feb 15;151(4):dev202231. doi: 10.1242/dev.202231.
37. Park SH, Lee SE, Jeon JH, Lee JH, Itakura E, Chang S, Choi WH, Lee MJ.
Formation of aggresomes with hydrogel-like characteristics by proteasome inhibition
Biochim Biophys Acta Gene Regul Mech. 2023 Jun;1866(2):194932. doi: 10.1016/j.bbagrm.2023.194932.
★36. Chiba M, Yanagawa M, Oyama Y, Harada S, Nemoto T, Matsuura A, Itakura E
A novel autophagy inhibitor, bTBT, disturbs autophagosome formation.
Autophagy Rep. 2023 Apr 6;2(1):2194620.
35. Park SH, Lee SE, Jeon JH, Lee JH, Itakura E, Chang S, Choi WH, Lee MJ.
Formation of aggresomes with hydrogel-like characteristics by proteasome inhibition.
Biochim Biophys Acta Gene Regul Mech. 2023 Mar 28;1866(2):194932. doi: 10.1016/j.bbagrm.2023.194932. Online ahead of print.
★34. Tomihari A, Kiyota M, Matsuura A, Itakura E.
Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins.
Sci Rep. 2023 Mar 28;13(1):4680. doi: 10.1038/s41598-023-31104-x.
★33. Ishii S*, Chino H*, Ode KL, Kurikawa Y, Ueda HR, Matsuura A, Mizushima N, Itakura E.
CCPG1 recognizes endoplasmic reticulum luminal proteins for selective ER-phagy.
Mol Biol Cell. 2023 Apr 1;34(4):ar29. doi: 10.1091/mbc.E22-09-0432. Epub 2023 Feb 3.
★32. Date Y, Matsuura A, Itakura E.
Disruption of actin dynamics induces autophagy of the eukaryotic chaperonin TRiC/CCT.
Cell Death Discov. 2022 Jan 25;8(1):37. doi: 10.1038/s41420-022-00828-6.
★31. Tomihari A*, Chiba M*, Matsuura A, Itakura E.
Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells.
STAR Protoc. 2021 Nov 23;2(4):100975. doi: 10.1016/j.xpro.2021.100975.
★30. Uesugi R, Ishii S, Matsuura A, Itakura E.
Labeling and measuring stressed mitochondria using a PINK1-based ratiometric fluorescent sensor.
J Biol Chem. 2021 Nov;297(5):101279. doi: 10.1016/j.jbc.2021.101279.
★29. Itakura E#, Chiba M, Murata T, Matsuura A. (#single corresponding author)
Heparan sulfate is a clearance receptor for aberrant extracellular proteins.
J Cell Biol. 2020 Mar 2;219(3). pii: e201911126. doi: 10.1083/jcb.201911126.
28. Otsubo K, Maeyashiki C, Nibe Y, Tamura A, Aonuma E, Matsuda H, Kobayashi M, Onizawa M, Nemoto Y, Nagaishi T, Okamoto R, Tsuchiya K, Nakamura T, Torii S, Itakura E, Watanabe M, Oshima S.
Receptor-Interacting Protein Kinase 3 (RIPK3) inhibits autophagic flux during necroptosis in intestinal epithelial cells.
FEBS Lett. 2020 Jan 29. doi: 10.1002/1873-3468.13748.
★27. Ishii S, Matsuura A, Itakura E.
Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe.
Sci Rep. 2019 Aug 12;9(1):11635. doi: 10.1038/s41598-019-48131-2.
26. Miura A, Itakura E, Matsuura A.
Reversible DNA damage checkpoint activation at the presenescent stage in telomerase-deficient cells of Saccharomyces cerevisiae.
Genes Cells. 2019 Aug;24(8):546-558. doi: 10.1111/gtc.12706. Epub 2019 Jun 17.
25. Takahashi K, Itakura E, Takano K, Endo T.
DA-Raf, a dominant-negative regulator of the Ras-ERK pathway, is essential for skeletal myocyte differentiation including myoblast fusion and apoptosis.
Exp Cell Res. 2019 Mar 15;376(2):168-180.
★24. Tatsumi T, Takayama K, Ishii S, Yamamoto A, Hara T, Minami N, Miyasaka N, Kubota T, Matsuura A, Itakura E# & Tsukamoto S# ( #co-corresponding authors)
Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse
Development 2018 Feb 23;145(4). pii: dev161893.
23. Takeda E, Jin N, Itakura E, Kira S, Kamada Y, Weisman LS, Noda T, Matsuura A.
Vacuole-mediated selective regulation of TORC1-Sch9 signaling following oxidative stress.
Mol Biol Cell. 2018 Feb 15;29(4):510-522.
22. Itakura E#, Chen C, de Bono M# ( #co-corresponding authors)
Purification of FLAG-tagged Secreted Proteins from Mammalian Cells.
Bio Protoc. 2017 Aug 5;7(15). pii: e2430.
★21. Takayama K, Matsuura A, Itakura E
Dissection of ubiquitinated protein degradation by basal autophagy
FEBS Lett. 2017 May;591(9):1199-1211 (selected as an Editor’s Choice)
20. Chen C, Itakura E, Nelson GM, Sheng M, Laurent P, Fenk LA, Butcher RA, Hegde RS and de Bono M
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
Nature 2017 Feb 2;542(7639):43-48
19. Yoshii SR, Kuma A, Akashi T, Hara T, Yamamoto A, Kurikawa Y, Itakura E, Tsukamoto S, Shitara H, Eishi Y, Mizushima N.
Systemic Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons.
Dev Cell. 2016 Oct 10;39(1):116-130
★18. Itakura E, Zavodszky E, Shao S, Wohlever ML, Keenan RJ, and Hegde RS
Ubiquilins chaperone and triage mitochondrial membrane proteins for degradation
Molecular Cell. 2016 Jul 7;63(1):21-33
17. Satpute-Krishnan P, Ajinkya M, Bhat S, Itakura E, Hegde RS, Lippincott-Schwartz J.
ER Stress-Induced Clearance of Misfolded GPI-Anchored Proteins via the Secretory Pathway.
Cell. 2014 Jul 31;158(3):522-33.
16. Kishi-Itakura C, Koyama-Honda I, Itakura E, Mizushima N.
Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells.
J Cell Sci. 2014 Sep 15;127(Pt 18):4089-102.
15. Ogasawara Y, Itakura E, Kono N, Mizushima N, Arai H, Nara A, Mizukami T,
Yamamoto A.
Stearoyl-CoA desaturase 1 activity is required for autophagosome formation.
J Biol Chem. 2014 Aug 22;289(34):23938-50.
14. Chen C, Itakura E, Weber KP, Hegde RS, de Bono M.
ODR-8, the C. elegans ortholog of Ufm1 specific protease 2, promotes ER maturation of G-protein coupled receptors by a non-catalytic, Ufm1-independent mechanism.
Plos Genetics 2014 Mar 6;10(3):e1004082.
13. Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, Mizushima N.
The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Mol Biol Cell. 2014 Apr;25(8):1327-37.
12. Sahani MH, Itakura E, Mizushima N.
Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids.
Autophagy. 2014 Mar;10(3):431-41.
11. Koyama-Honda I, Itakura E, Fujiwara TK, Mizushima N.
Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site.
Autophagy. 2013 Jul 10;9(10). 1491-1499
★10. Itakura E, Kishi-Itakura C, Mizushima N.
The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Cell. 2012 Dec 7;151(6):1256-69.
9. Osuna M, Sonobe Y, Itakura E, Devnath S, Kato T, Kato Y, Inoue K.
Differentiation capacity of native pituitary folliculo-stellate cells and brain astrocytes.
J Endocrinol. 2012 Jun;213(3):231-7.
★8. Itakura E, Kishi-Itakura C, Koyama-Honda I, Mizushima N.
Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy.
J Cell Sci. 2012 Mar 15;125 : 1488-99
★7. Itakura E, Mizushima N.
p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding.
J Cell Biol. 2011 Jan 10;192(1):17-27.
★6. Itakura E, Mizushima N.
Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins.
Autophagy. 2010 Aug 16;6(6):764-76.
★5. Itakura E, Kishi C, Inoue K, Mizushima N.
Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG.
Mol Biol Cell. 2008 Dec; 19(12): 5360-72.
★4. Itakura E, Odaira K, Yokoyama K, Osuna M, Hara T, Inoue K.
Generation of transgenic rats expressing green fluorescent protein in S-100beta-producing pituitary folliculo-stellate cells and brain astrocytes.
Endocrinology. 2007, 148(4): 1518-23
★3. Itakura E, Sawada I, Matsuura A.
Dimerization of the ATRIP protein through the Coiled-Coil Motif and its implication of the Maintenance of Stelled Replication Forks.
Mol. Biol. Cell. 2005, 16(12):5551-62
★2. Itakura E, Takai K, Umeda K, Kimura M, Oshumi M, Tamai K, Matsuura A.
Amino-terminal domain of ATRIP contributes to intranuclear relocation of the ATR-ATRIP complex following DNA damage.
FEBS Lett. 2004, 577(1-2):289-93.
★1. Itakura E, Umeda K, Sekoguchi E, Takata H, Ohsumi M, Matsuura A.
ATR-dependent phosphorylation of ATRIP in response to genotoxic stress.
Biochem, Biophys, Res. Commun. 2004, 323(4):1197-202.
Reviews
Klionsky DJ, … Itakura E, et al.,…
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
Autophagy. 2021 Jan;17(1):1-382.
Klionsky DJ, … Itakura E, et al.,…
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Autophagy. 2016 Jan 2;12(1):1-222. (review)
Itakura E, Mizushima N.
Syntaxin 17: The autophagosomal SNARE.
Autophagy. 2013 Mar 6;9(6). 917-919 (Review)
Itakura E, Mizushima N.
Atg14 and UVRAG: Mutually exclusive subunits of mammalian Beclin 1-PI3K complexes.
Autophagy. 2009 May 2;5(4): 534-536 (Review)